The transfected DCs (1 105) were washed and cocultured with HLA-A2-positive T cells (1 106?cell?ml?1) from your same donor in 24-well plates (Falcon, USA). Cells were subcultured twice a week with complete medium comprising IL-2 (20?U?ml?1) and IL-7 (10?ng?ml?1; Peprotech, UK). The irradiated transfected DCs were added to the plates every full week. After incubation… Continue reading The transfected DCs (1 105) were washed and cocultured with HLA-A2-positive