Supplementary MaterialsMethods S1: (0. harvested at 6 hpi and analyzed for expression of the GFP transgene using Western blotting. Tubulin was probed as a loading control. This experiment was completed in triplicate with similar results. (B) RNA was also prepared from both liver and spleen tissues and analyzed for the presence of GFP and rEA… Continue reading Supplementary MaterialsMethods S1: (0. harvested at 6 hpi and analyzed for