Supplementary MaterialsSupplemental data jciinsight-3-121109-s191. and using established antibodies to isolate microglia and oligodendrocytes, we document that these 4 major cell types are isolated with high purity and RNA quality. We validated our procedure by performing acute peripheral LPS challenge, while highlighting the underappreciated changes occurring in astrocytes and vascular endothelia in addition to microglia. Furthermore,… Continue reading Supplementary MaterialsSupplemental data jciinsight-3-121109-s191. and using established antibodies to isolate microglia