Affinity purification followed by enzymatic digestion and mass spectrometry has been widely utilized for the sensitive detection of interacting proteins and protein complexes in various organisms. 12 co-immunoprecipitated protein subunits in Pol V complex and to discover 17 potential interacting protein targets in glycine-HCl), or sodium dodecyl sulfate (SDS) detergent solution and SDS-PAGE separation. However,… Continue reading Affinity purification followed by enzymatic digestion and mass spectrometry has been