Application of BIOMED-2 standardized immunoglobulin (Ig) gene rearrangement system is of significance for MM diagnosis. Keywords: Multiple myeloma, Polymerase chain reaction, Fragment analysis, BIOMED-2 THBS1 standardized Ig gene rearrangement system multiple myeloma, MMImmunoglobulin, Bromodomain IN-1 IgMIgBB[1]PCRMMBIOMED-2IgIgMM 120092013167MM20Reactive plasmacytosisRP2008WHO 2DNADNA[]DNAThermo ScientificDNADNA Bromodomain IN-1 3PCRInvivoscribeIgH VH-JHIgH DH-JHIgKPCR95 Bromodomain IN-1 C7 min, 95 C 45 s60 C 45 s72 C 90 s3572 C10 min 4PCR1 l10 l0.1 l GeneS-can-500 LIZApplied Biosystems95 C 2 min4 C 5 min3730Applied BiosystemsPCR[1][2] 5FISHIgHVysisThermoBriteAbbottIsisMetasystems500 6SPSS 17.02P<0.05 1DNADNA50 ng/lDNAA260/ A2801.8~1.9DNADNA 2167MMIg167MMIgH VH-JH107IgH VH-JH FR1FR2FR387803460IgH DH-JH14.0%15/10730.0%18/60P=0.013167MMIgK12172.4%IgK Bromodomain IN-1 VK-JKVK/intronRSS-Kde9182IgH VH-JHIgH DH-JHIgK94.6%158/167 3167MM86IgG44IgA2019IgD 3IgMMIgGIgAIgGIgAIgDMMIgHIgKP>0.05MMIgHIgKP=0.0191 1 Ig[]
IgHIgKPIgG4437(84.1)32(72.7)0.195IgA2018(90.0)13(65.5)0.1271911(57.9)18(94.7)0.019IgD32(66.7)2(66.7)1.000 Open in a separate window 4167IgH95.4%IgH1 Open in a separate window 1 MMIgH VH-JH FR1ABMMarker12MM 5IgFISH IgH53MMIgFISH IgHIgH67.9%36/5349.1%26/53P=0.049FISH IgHIg5IgK 6RPIgRPIgH VH-JHIgH DH-JHIgK MMBBBIgHVHDHJHVDJIgHIgBPro-B-cellDH-JHBPre-B-cellVHDJHVH-JHVH-JHDH-JHBCDRB[3]VH-JHIgKSomatic hypermutation, SHMClass switch recombination, CSRMMIgVHIgHIgGIgA[4]SHMIgH VHSHMIgH VHSHMPCRMMVH-DJHB9%[5]C[6]MMIGH VH-DJHIgH DH-JHSHMIgH VH-JH30.0IgH DH-JH14.0%P=0.013MMIgH DH-JHMRD[7] IgKBVK-JKIgKVK-Kde[7]IgHSHMIgKSHMIgH[8]167MMIgIgH+IgK94.6%B IgMMIgGIgAIgAMMIgHIgKP=0.127MMIgKIgHP=0.019IgMM IgMMPCRPCR34PCRPCR1[1]IgKIgKKdeintronRSSIgK VK-KdeintronRSS-Kde MMIgH13p531q21[9]IgHMM[10]Fonseca[11]IgHMMFISHFISH Bromodomain IN-1 IgHPCR53MMIgIgHIgH67.949.1%P=0.04926FISH IgHIg5IgKSHMIgH VH-JHBIOMED-2IgFISH IgH MMIgHIgK94.6%RPIgBIOMED-2IgMMMM Funding Statement 81400175. MMIgHIgK94.6%RPIgBIOMED-2IgMMMM Funding Statement 81400175.